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ISSN : 1598-5504(Print)
ISSN : 2383-8272(Online)
Journal of Agriculture & Life Science Vol.47 No.5 pp.29-41
DOI :

Phylogenetic Analysis on the Seven Species of Theaceae Growing in Korea Based on RAPD and ITS Sequences of Nuclear Ribosomal DNA

Gab-chul Choo2*, Jong-bin An1, Jeong-geun Park1
2Department of Forest Resources, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea
1Korea National Arboretum, Pocheon 487-821, Korea
Received: JUN. 12. 2013, Revised: SEP. 4. 2013, Accepted: OCT. 7. 2013

Abstract

The genetic relationships among five genera, seven species of Theaceae were examined througha random amplified polymorphic DNA (RAPD) analysis and internal transcribed spacer (ITS). InRAPD analysis, five of 15 arbitrary primers showed polymorphic bands, which were able toclassify different genera and species of Theaceae. The genetic variations of Theaceae were from0.031 to 0.484. In ITS analysis, the ITS sequences were analyzed using BLAST and showed highidentities with sequences of Theaceae, seven species published in NCBI GenBank database, whichranged from 98 to 100%. Sequence alignment of seven species showed 34.9% identities for ITS 1region and 43.7% for ITS 2 region. Pairwise sequence divergences among seven species rangedfrom 0 to 0.330%. In phylogenetic tree, they were divided into three groups. In conclusion, themolecular data generated in the present investigation will help to understand the geneticrelationships of Theaceae and also might be useful for further studies in intra-species, inter-species,and molecular evolution researches.

Reference

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